利用甲醇厂所排放的高浓度CO2尾气培养小球藻,研究不同的通气方式、培养方式对小球藻生长的影响。实验结果表明,当通气速率为300 mL/min、CO2浓度为10%并以10 min/h的间歇通气方式培养时能够提高小球藻的生物量,生物量和产率分别为0.681 g/L和0.082 g/(L.d)。对氮源和磷源采用补料的方式进行培养时对小球藻生长具有促进作用,最高生物量和产率分别达到0.789 g/L和0.088 g/(L.d)。当培养基的更新率为20%时能够获得较高的生物量。利用醋酸进行兼性培养时,一次性添加50～125μL/L醋酸都可促进小球藻生长,每日添加10～25μL/L的醋酸时,可明显促进小球藻生长,最高生物量和产率分别为0.933 g/L和0.111 g/(L.d),分别是空气对照的1.3倍和1.4倍。说明利用甲醇厂高浓度CO2尾气培养小球藻提高生物量是可行的。

Use flue gas derived from a methanol plant which contains high concentration of carbon dioxide to cultivate Chlorella sp.,the effects of different aeration modes and cultivation modes on the growth of Chlorella sp.were determined.The results indicated that the microalgae biomass increased when flow rate was at 300 mL/min of 10% CO2 per min and the flow frequency was 10 min/h,the microalgae dry mass and biomass productivity were 0.716g/L and 0.088 gg/(L·d),respectively.The biomass concentration obtained in fed-batch cultivation with nitrate and phosphorus feeding was higher than that obtained in batch cultivation,the maximize microalgae dry mass and biomass productivity were 0.746g/L and 0.093g/(L·d).Respectively.The higher microalgae biomass was obtained when the renewal rate was 20%.When mixotrophic culture with acetic acid,while acetic acid(concentration from 50μL/L to 125μL/L) was added to the culture medium,it could accelerate the growth of Chlorella sp..When acetic acid(concentration from 10μL/L to 25μL/L)was added to the culture medium daily,higher growth rates were obtained than that in normal conditions,and the maximize microalgae dry mass and biomass productivity were 0.986g/L and 0.119g/(L·d),respectively,which were 1.3 times and 1.4 times higher than that of the controls.The study shows that using flue gas derived from a methanol plant to enhance the microalgae biomass of Chlorella sp.is feasible.