为进一步降低成本把原料啤酒糟加工成优质的水产饲料,以白酒丟糟作为辅料,自组装木霉好氧发酵装置,用Plackett-Burman与响应曲面法对绿色木霉半固态降解混糟的工艺进行了优化,并用光合菌二次发酵转化提高混糟蛋白质含量。当啤酒干糟与白酒丢干糟之比为2.67∶1(g∶g)、料水比为1∶2.88(干混糟g∶水g)、接种量为9.5%(w/w)、发酵初始pH为5.0、发酵前期温度30℃维持4.82d、发酵后期50℃维持2.5d、料层厚度为9cm、通风量83.2 mL/min时,粗纤维素含量可从17.2%(w/w)下降至9.9%(w/w);经光合菌二次发酵后,干混糟粗蛋白含量可从21.5%上升至43.9%、真蛋白含量从16.1%(w/w)上升至38.4%(w/w)、粗纤维素从17.2%(w/w)下降至5.9%(w/w)、粗脂肪从5.7%(w/w)上升至8.1%(w/w)、粗灰分从6.6%(w/w)上升至7.8%(w/w)、总磷从0.6%(w/w)上升至1.3%(w/w)、发酵干糟含水率为9.8%(w/w),这6项指标达到了"中华人民共和国水产行业标准"的罗非鱼鱼种饲料标准(SC/T1025-2004)。

In order to make the low-cost and high-quality feed from brewers' spent grains,using the distillers' grains as an auxiliary material,the fermentation technology of Trichoderma viride was investigated with an aerobic fermentation installation and with the methods of Plackett-Burman and response surface.The fermentation of Rhodopseudomonas palustri was adopted to increase the content of protein after Trichoderma viride fermentation.With the ratio of dried brewer grains-to-dried distillers' grains set at 2.67∶ 1(w/w),the mixed grains-to-water ratio set at 1∶ 2.88(w/w),the inoculum concentration at 9.5 %(w/w),the initial pH at 5.0,the early fermentation at 30℃ for 4.82 d,the later fermentation at 50℃ for 2.5d,the thickness of feedstock layer at 9 cm,and the air volume at 83.2 mL/min,the crude cellulose content of mixed grains was decreased from 17.2 to 9.9 %(w/w).After the second fermentation of Rhodopseudomonas palustri,the true protein content of mixed grains was increased from 16.1% to 38.4%(w/w),the content of crude protein was increased from 21.5% to 43.9%,the crude cellulose content of mixed grains was decreased from 17.2% to 5.9%(w/w),the fat content of mixed grains was increased from 5.7% to 8.1%(w/w),the crude ash content of mixed grains was increased from 6.6% to 7.8%(w/w),the total phosphorus content of mixed grains was increased from 0.6% to 1.3%(w/w),and the water content of the grains was 9.8%(w/w).The six indexes satisfied the China standard for tilapia's fingerling(SC/T1025-2004).