蔷薇藻(Rhodella reticulata)是属于红藻门的一种海洋单细胞微藻,在生长过程中会产生藻胆蛋白、胞外多糖等生物活性物质。文中研究了蔷薇藻的藻蓝蛋白的分离纯化及抗氧化性能,藻细胞经过破碎,通过硫酸铵沉淀和DEAE Sepharose FF柱层析后,可以分离出较纯的藻蓝蛋白,纯化的藻蓝蛋白在620 nm处有一个最高的吸收峰,纯度为3.92,经SDS-PAGE电泳后,测得藻蓝蛋白含有α和β两个亚基,分子质量分别为18 ku和22 ku。蔷薇藻藻蓝蛋白由17种氨基酸组成。蔷薇藻纯化后的藻蓝蛋白比粗藻蓝蛋白具有更高的抗氧化能力,且呈剂量依赖效应。

The unicellular red alga,Rhodella reticulata,can synthesize some bioactive substances such as phycobiliprotein and exopolysaccharides.In this paper,isolation and purification of phycocyanin from Rhodella reticulate and its characteristic were studied.The purer phycocyanin was precipitated by(NH4)2SO4 and then purified using DEAE-Sepharose Fast Flow ion exchange column.The purified phycocyanin had an absorbance maximum at 620nm and its purity reached 3.92.Using SDS-PAGE,the molecular mass of intact C-phycocyanin was estimated to be 18 ku and 22 ku with α and β subunits forming dimmers.The phycocyanin of Rhodella reticulate had 17 kinds of amino acid.The antioxidant activity of pure phycocyanin of Rhodella reticulate was higher than that of crude phycocyanin and showed a dose-dependent manner.