为了研究茶多糖不同组分对抗凝血活性的影响,通过水提醇沉的方法对茶多糖进行提取,经聚酰胺柱脱色、脱蛋白,采用离子交换柱DEAE Sepharose CL-6B对多糖进行分离纯化,用高效凝胶渗透色谱测定其分子量,并对多糖组分抗凝血活性进行了研究。结果表明:聚酰胺法脱色、脱蛋白效果较好,脱色率、蛋白去除率和多糖保留率分别为75.1%、91.2%和79.2%;经纯化后得到4个组分,分别为TPS-1、TPS-2、TPS-3和TPS-4;HPGPC法测定TPS-1、TPS-2和TPS-3分子量分别为20760、24230和250643,为均一多糖,TPS-4含两个组分,其分子量分别为689 113和4 150,为非均一多糖。体外抗凝血实验显示TPS-4能延长活化部分凝血活酶时间(APTT)和凝血酶时间(TT),说明是通过内源性途径来影响凝血的。

To study different components of tea polysaccharides and their anticoagulant activity,the tea polysaccharides were extracted by water and precipitated by alcohol,and purified by ion exchange chromatograph on DEAE sepharose CL-6B column,and its molecular weight was determined by high performance gel permeation chromatograph.The anticoagulant activity of polysaccharide was also studied.The results showed polyamide method was effective on decolorization and deproteination,the decolorization and deproteination and polysaccharides retention rate were 75.5%,91.2% and 79.2% respectively;four componets were obtained after purification: TPS-1,TPS-2,TPS-3 and TPS-4.HPGPC method showed that the TPS-1,TPS-2 and TPS-3 were homogeneous polysaccharides.Their molecular weight were 20760、24230 and 250643,separately,they are homogeneous.TPS-4 has two components of molecular weight of 689113 and 4150,and it was heterogeneous.Anticoagulant activities in vitro tests showed TPS-4 could prolong activated partial thromboplastin time(APTT) and thrombin time(TT).The result indicated TPS in the regulation of coagulation initiated via the intrinsic pathway.