利用Sephadex LH-20凝胶分离纯化油茶籽壳中的原花青素,并对洗脱后得到的样品进行分析鉴定。结果表明:采用60%的丙酮溶液洗脱得到的原花青素含量较高,可达57.3%;HPLC分析显示,从Sephadex LH-20洗脱下来的组分中含有原花青素的单体物质。

The procyanidins from the tea(Camellia oleifer) Seed Shell was isolated and purified with Sephadex LH-20,and the samples were analyzed and identified.The result showed that procyanidins which from the tea(Camellia oleifer) Seed Shell,was purified by Sephadex LH-20 using 60% acetone elution received higher levels of procyanidins,the yield was 57.3%.The purified product was further analyzed by HPLC.It indicated that the Procyanidin monomer was existed in the samples.