运用响应曲面法优北化五味子多糖超声辅助乙醇提取条件,并对其抗氧化性能进行了研究。结果表明:乙醇浓度,料液比,提取温度对北五味子多糖提取率有显著影响,采用Box-Behnken响应面技术得到最佳提取条件为:乙醇体积分数(X1)=54.7%、料液比(X2)=1∶25、提取温度(X3)=58℃;其他条件为:超声功率200W,提取时间40 min,提取次数2次。在此条件下,北五味子多糖相对提取率为87.12%(n=3),优于传统的水提法和醇提法。北五味子多糖具有较强的抗氧化性能。

The ultrasound-assisted extraction condition of polysaccharides from Schisandra chinensis(Turcz.)Baill fruits(SCP) was optimized by Response Surface Methodology(RSM).The results showed that the significant factors of SCP extraction were ethanol concentration,ratio of solid to liquid and extraction temperature.The optimal extraction conditions obtained by RSM were as follows: ethanol concentration(X1) 54.7%,solid to liquid ratio(X2) 1∶ 25,extraction temperature(X3) 58℃.The other conditions were ultrasonic power 200W,extraction time 40min and extraction twice.Under the optimal extraction conditions,the relative extraction rate of SCP could reach to 87.12%(n=3).The ultrasound-assisted extraction was better than traditional method.The Antioxidant activities of SCP on ·OH and ·O2-were determined by spectrophotometry.The results showed that SCP had strong antioxidant activities.