运用柱前衍生-高效液相色谱法测定暗纹东方鲀肉中牛磺酸的含量,以邻苯二甲醛为衍生剂,样品液与衍生剂进行衍生反应的体积比为1∶1,反应时间为1 min,甲醇-乙腈-水(体积比10∶20∶70)为流动相,采用XbridgeTMC18色谱柱,波长为330 nm,流速1 mL/min,进样量20μL的条件下检测样品中的牛磺酸。结果表明:牛磺酸的线性范围为5～30μg/mL,相关系数R2为0.999 1,回收率范围95.35%～98.19%,变异系数(RSD)=2.03%～3.71%(n=3),样品6次重复测定的RSD=0.51%,最小检出限1.14μg/mL。

A pre-column derivatization high-performance liquid chromatography(HPLC) method for the determination of taurine in Takifugu obscurus was developed.Taurine was derivatized with Ophthalaldehyde(OPA).The derivatives were separated on a reversed-phase XbridgeTM C18 column by a mobile phase by(A) methanol,(B) acetonitrile and(C) water(10/20/70).Sample size was about 20 μL,flow rate was about 1 mL/min and detection was done at 330 nm by ultraviolet detector.Results showed that the linear ranger of taurine was 5~30μg/mL,correlation coefficient R2=0.9991,the recoveries were 95.35%~98.19%,RSD=2.03~3.71%(n=3).The precision RSD = 0.51%(n=6) and the detection limit was1.14μg/mL.The method is simple,quick,specific,sensitive,repetitively and accurate.