对缺陷短波单胞菌(Brevndimonas diminut)JNPP-NSS产L-脯氨酸的补料分批发酵条件进行了研究。结果表明,发酵初始添加50 g/L前体物质谷氨酸,以最适初始葡萄糖浓度100 g/L,于35 h以3.0 g/(L.h)的流速补加葡萄糖,使总糖浓度达120 g/L的补碳方式,L-脯氨酸的合成浓度有所提高。发酵结束,L-脯氨酸的终浓度提高到54.40 g/L,底物葡萄糖对L-脯氨酸的转化率达到0.45 g/g。

Fed-batch fermentation conditions of Brevndimonas diminut JNPP-NSS for L-proline production were studied.The results showed that the synthetic concentration of the L-proline was improved under the conditions as follows: the initial precursor glutamic acid concentration was 50 g/L,the optimal original glucose concentration was 100 g/L,the glucose was fed with 3.0 g/(L·h) at 35 h,and the total glucose concentration was 120 g/L.As a result,the concentration of L-proline increased to 54.40 g/L with a yield of 0.45 g/g.