采用响应面分析法(response surface analysis,RSA)优化了微波浸提低温豆粕水溶性多糖的工艺条件。在单因素实验结果基础上,选取pH值、时间、料水比及微波功率4个因素进行中心组合旋转实验设计(central composite rotatable design,CCRD),并通过RSA对微波浸提水溶性大豆多糖工艺参数进行了优化组合。结果表明:在pH 8.0,料水比(g∶mL)1∶6,提取时间2.6 min,功率380W条件下,水溶性大豆多糖的得率可达5.86%,与验证实验相对误差为0.44%,证明响应面法优化结果是真实有效的。

Microwave extraction technology of soluble soybean polysaccharides(SSPS) from low-temperature soybean meal was optimized by response surface analysis.Based on the single-factor experiments,four independent variables(pH,ratio of water to raw material,time and microwave power) were chosen to design central composite rotatable experiments.Furthermore,the combination of four parameters for microwave extraction of SSPS was obtained.The experiment results showed that the optimum condition was: pH 6.4,ratio of water to material 5 mL/g,time 2min and microwave power 380 W5.Under the above conditions,SSPS yield was achieved 86%.The relative standard difference was only 0.44% between the actual and predictive value of SSPS yield.Hence,the RSA optimization is very effective for microwave extraction technology of SSPS.