研究了蜂花粉与蜂王浆中葡萄糖、果糖、蔗糖和麦芽糖的离子色谱分析方法。样品用热水溶解,乙腈沉降蛋白,使用2个固相萃取小柱去除色素与脂肪等干扰成分后,以CarboPac PA10分析柱为色谱柱,氢氧化钠和乙酸钠溶液为流动相进行离子色谱分离。葡萄糖、果糖、蔗糖和麦芽糖的检出限分别为0.12,0.14,0.21,0.33mg/L。蜂花粉的回收率93.5%～102.2%,精密度为4.4%～6.3%,蜂王浆的回收率为95.0%～103.5%,精密度为6.7%～10.2%。利用该方法对20个蜂花粉和20个蜂王浆样品进行了分析,花粉中葡萄糖含量为9.9%～20.6%,果糖14.2%～26.9%,蔗糖1.8%～8.3%,麦芽糖0.33%～1.4%;王浆中葡萄糖含量为6.2%～8.3%,果糖7.0%～8.7%,蔗糖0.38%～3.6%,麦芽糖0.27%～0.83%。该方法简单、灵敏度高,可作为标准方法对蜂花粉与蜂王浆中葡萄糖、果糖、蔗糖和麦芽糖进行分析。

An ion chromatography method was developed for determination of sugars in bee pollen and royal jelly.Sugars were extracted with hot water and cleaned up by solid phase extraction after protein precipitation by acetonitrile.Sugars were separated on a Carbora PA10 column with NaOH/ NaOAc solution as mobile phase.The limit detection of glucose,fructose,sucrose and maltose were 0.12,0.14,0.21,0.33 mg/L,respectively.The relative standard deviations of bee pollen were 4.4%~6.3% and the recoveries are 93.5%~102.2%,the relative standard deviations of royal jelly were 6.7%~10.2% and the recoveries were 95.0%~103.5%.The method was applied for the analysis of 20 bee pollen samples and 20 royal jelly samples.The average concentrations of glucose,fructose,sucrose and maltose in bee pollen samples were 9.9~20.6%,14.2~26.9%,1.8%~8.3%,0.33%~1.4%,respectively.The average concentrations of glucose,fructose,sucrose and maltose in royal jelly samples were 6.2~8.3%,7.0~8.7%,0.38%~3.6%,0.27%~0.83%,respectively.The method is simple and suitable for a routine quality sugar control of bee pollen and royal jelly.