食品与发酵工业

酒醅中纤维素酶产生菌的生理生化特性及与其产酶活力的相关性

  • 郭建华 ,
  • 刘冀 ,
  • 郭宏文 ,
  • 刘晓兰 ,
  • 贾士儒
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网络出版日期: 2012-11-25

Physiological and Biochemical Characteristics of Cellulase Producing Bacteria from Fermented Grains and Correlation with the Cellulase Activity

  • Guo Jian-hua . ,
  • Liu Ji ,
  • Guo Hong-wen ,
  • Liu Xian-lan ,
  • Jia Shi-ru
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Online published: 2012-11-25

摘要

以白酒酒醅中筛选出的产纤维素酶的细菌为研究对象,测定了这些细菌的细胞长宽比、糖发酵实验、淀粉水解实验等生理生化特性指标和它们产纤维素酶活力的大小,并利用相关分析和主成分分析研究了生理生化特性指标与纤维素酶活力之间的关系。相关分析结果表明,纤维素酶产生菌的各种生理生化特性指标之间,及这些生理生化特性指标与细菌产纤维素酶活力之间均存在着不同程度的相关性。主成分分析结果表明,纤维素酶活力与细胞长宽比和利用葡萄糖产酸之间存在着明显的负相关性,而与其淀粉水解能力、利用乳糖产酸和柠檬酸盐试验指标之间存在明显的正相关性。

本文引用格式

郭建华 , 刘冀 , 郭宏文 , 刘晓兰 , 贾士儒 . 酒醅中纤维素酶产生菌的生理生化特性及与其产酶活力的相关性[J]. 食品与发酵工业, 2012 , 38(11) : 11 -15 . DOI: 10.13995/j.cnki.11-1802/ts.2012.11.009

Abstract

Using cellulase producing bacteria from liquor fermented grains as study object,physiological and biochemical characteristics such as cell aspect ratio,sugar fermentation experiments,starch hydrolysis experiments,and cellulase activity of cellulase producing bacteria was measured,and a correlation between the physiological and biochemical characteristics and cellulose enzyme activity was studied using correlation analysis and principal component analysis.The results of correlation analysis indicated that there were different degrees of correlation among indicators of physiological and biochemical characteristics,and between cellulase activity of cellulase producing bacteria and indicators of physiological and biochemical characteristics.The results of principal component analysis indicated that cellulase activity had a clear negative correlation with cell aspect ratio and producing acid using glucose,while had a positive correlation with starch hydrolysis,producing acid using lactose and citrate test indicators.
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