通过对4种常见的包埋材料固定化克雷伯氏菌发酵生产1,3-丙二醇的研究发现,海藻酸钙固定化无论在制备难易还是在发酵强度上都有很大优势,并进一步确定了海藻酸钙固定化的最优条件:海藻酸钠质量浓度6%,CaCl2质量浓度4%,交联时间4 h。相对于游离发酵,固定化发酵1,3-丙二醇终浓度提高了7.4%,发酵强度达到了1.04 g/(L.h)。此外,固定化克雷伯氏菌还能用于1,3-丙二醇半连续发酵。

1,3-propanediol fermentation by immobilized Klebsiella pneumonia was studied.The calcium alginate was found that it has great advantages in the microcapsules preparation and 1,3-propanediol productivity by the comparison study of four kinds of common embedding materials.The optimum conditions of calcium alginate encapsulation were sodium alginate of 6%(w/v),calcium chloride of 4%(w/v),crosslinking time of 4 h.The concentration of 1,3-propanediol in the broth increased by 7.4% compared with free fermentation,and the productivity of 1,3-propanediol reached 1.04 g/(L·h).Moreover,the immobilized Klebsiella pneumoniae can be used for semi continuous 1,3-propanediol fermentation.