对香柚籽中柠檬苦素的提取及纯化工艺进行了优化。通过实验得到优化提取条件为:溶剂乙醇体积分数70%、提取温度70℃、提取时间2 h、料液比1∶28;适宜吸附流速为0.75 mL/min;以体积分数80%的乙醇作为洗脱剂,最佳的洗脱流速为0.5 mL/min。

The extraction and purification of limonin from pomelo seeds were studied.First,the effect of major single factors on the extraction of Pomelo was investigated.Then the extraction conditions were optimized by orthogonal experimental design.The optimum extraction conditions were: 70% ethanol concentration,70℃ of extraction temperature,2 hour of pretreatment,1:28 ratio of material and solvent.The best adsorption flow rate was 0.75 mL/min;the best elution flow rate was 0.5mL/min with 80% ethanol as eluting agent.