以1株自行分离的菠萝果酒酵母菌H10为出发菌株,制备原生质体后进行紫外诱变选育优良突变菌株。优化了酶法制备菠萝果酒酵母原生质体的工艺条件和紫外照射时间,并对诱变菌株进行初筛和复筛。结果表明:菌龄10 h、在酶解温度32℃、酶解液pH6.2、酶解时间90 min为原生质体制备的最佳工艺条件;最优紫外灯照射时间为150 s;诱变菌株经过初筛和复筛后,得到1株对菠萝汁发酵能力强,产香能力好,耐乙醇和SO2,遗传稳定性好,适合热带地区菠萝酒生产的的优良酵母菌株H10-15。

In this research,protoplast was prepared with the initial wine yeast of a kind of automatic separated pineapple yeast H10,which was used to make UV mutagenesis to breed fine mutant strain.Process conditions and Ultraviolet irradiation time of yeast protoplast of customize pineapple wine were studied and mutant strains were primarily and secondly screened.The results had shown that a cell age of 10h,a enzymolysis temperature of 32℃,a enzymatic hydrolysate pH of 6.2 and a enzyme digestion time of 90min were the best process conditions for protoplast preparing.The best Ultraviolet irradiation time was 150s.After primary and second screens of mutant strains,a fine yeast mutant strain NO H10-15 which suits tropical pineapple wine production with good fermentability,aroma producing ability,alcohol and SO2 resistance and genetic stability will be produced.