以姜蓉中分离的栖冷克吕沃尔菌为对象,研究该菌还原硝酸盐及其在不同冷藏温度、NaCl浓度和pH值下的繁殖情况,以该菌污染姜蓉,研究其在亚硝酸盐产生中发挥的作用。结果表明:栖冷克吕沃尔菌能还原硝酸盐,在1℃无明显生长,4℃可缓慢生长,8℃生长迅速,在NaCl浓度为0.5%、1%、2%时生长良好,在NaCl浓度为5%时逐渐死亡,该菌在pH5.0～7.0条件下适宜生长,pH4.0条件下逐渐死亡,栖冷克吕沃尔菌污染姜蓉4～8d后开始检出亚硝酸盐,当细菌浓度增长至108CFU/g时,多数姜蓉中亚硝酸盐含量达到峰值。

The nitrate reduction of Kluyvera cryocrescens isolated from ginger and its propagation at different temperature,concentration of sodium chloride,and pH valu was studied.The role of Kluyvera cryocrescens on nitrite generation of ginger was observed.The results showed that Kluyvera cryocrescens could reduce nitrate to nitrite.It did not propagate at 1℃,but propagated slowly at 4℃ and rapidly at 8℃.It propagated well in 0.5%,1%,2% sodium chloride medium,but died slowly in 5% sodium chloride medium.It propagated well at pH5.0~7.0 in culture medium,but died gradually at pH4.0.Nitrite could be detected in 4 to 8 days after gingers were contaminated by Kluyvera cryocrescens.Nitrite content was at peak when Kluyvera cryocrescens reached 108 CFU/g in most of gingers.