为确定块菌多糖水提适宜工艺条件,以块菌粗多糖得率为指标,根据Box-Benhnken的中心组合试验设计原理,在单因素试验的基础上采用三因素三水平的响应面法对提取工艺进行优化。结果表明:在温度为94.68℃,提取时间为3.71 h,液料比值58.66时提取率最高为10.8%。采用DPPH自由基清除法、羟基自由基清除法、还原力检测法对块菌粗多糖的抗氧化活性进行测定,结果表明块菌粗多糖具有较好的抗氧化活性。

In order to set up the optimization of water extraction technique of polysaccharides from truffles,the yield of crude polysaccharides was chosen as the index,based on the principle of Box-Benhnken Center combination experimental design,the optimization of extraction technique of polysaccharides was carried out by three factors on three levels.The results showed the highest extraction ratio(10.8%) was obtained at 94.68℃,extraction 3.71h,and Water-solid ratio of 58.66.Evaluation of antioxidant activity is measured by DPPH radical scavenging activity,Hydroxyl radical scavenging activity and Reducing power.The results indicate that crude polysaccharide of truffles has a good antioxidant activity.