以蛹虫草子实体为原料,通过碱法提取蛹虫草的蛋白质,以蛋白质的提取率作为提取工艺的衡量指标。在单因素实验的基础上,通过Box-Behnken模型对提取工艺进行响应曲面优化,在提取温度为60℃的条件下,确定最佳提取条件为:提取时间128 min,料液比为1∶16.2(g∶mL),NaOH溶液的浓度0.14 mol/L。在此条件下验证蛋白质的得率为88.92 mg/g与预测值88.19 mg/g接近。

Alkaline extraction method was used to extract protein from Cordyceps militaris.The protein yield was designed as the target index.Based on single-factor experiment,and the principle of Box-Behnken central composite design,response surface analysis(RSA) was applied to optimize the extraction conditions of protein from Cordyceps militaris.At the extracting temperature of 60℃,the optimum extraction conditions were: extrcating time 128 min,solid-liquid ratio 1∶ 16.2,NaOH solution concentration 1.4 mol/L.Under the above conditions,the highest protein yield of estimated and actual values were 88.19mg/g and 88.92%,respectively.