使用Protamex蛋白酶对克氏螯虾虾壳进行水解,研究虾壳蛋白多肽的还原能力和Fe2+螯合力,DPPH.清除能力,ABTS.+清除能力和羟基自由基清除能力。结果表明,虾壳蛋白肽的自由基清除能力和还原力均随着肽浓度的增大而增大。Fe2+螯合力同样与蛋白肽的浓度之间存在着量效关系,但是当浓度达到10 mg/mL时,螯合力不再继续增大。通过对蛋白肽的分子量分布进行研究发现,92.79%的多肽的分子质量小于1 000 u,其中21.50%的多肽分子质量小于180 u,50.51%的多肽分子质量为180～500 u,20.78%的多肽分子质量为500～1 000 u。

The antioxidant activities such as reducing power,Fe2+ chelating activity,DPPH·radical scavenging activity,ABTS·+ scavenging activity,and hydroxyl radical-scavenging activity of crayfish(Procambarus clarkii) shell protein hydrolysate(CSPH) prepared with Protamex were evaluated.The radical scavenging activity,reducing power and Fe2+ chelating activity of CSPH increased with its increasing concentration.The Fe2+ chelating activity was fixed when its concentration reached 10 mg/ml.The molecular weight distribution of CSPH revealed that 92.79% of the total amount was peptides with molecular weight lower than 1 000 u,composing mostly of low molecular weight peptides lower than 180 u(21.50%),from 180 to 500 u(50.51%),and from 500 to 1 000 u(20.15%).