通过研究6种大孔吸附树脂和2种离子交换树脂对银杏果多糖溶液色素脱除的影响,筛选出3种树脂:大孔阴离子交换树脂脱色1号、D900和非极性的大孔吸附树脂DA-201C。通过正交试验对脱色条件进行优化。在静态吸附试验研究的基础上,筛选出效果较好的树脂进行动态试验研究。结果表明:银杏果多糖中的色素可能以带负电荷的非极性小分子色素为主,在采用脱色1号树脂,pH值为4.5,温度为25℃,上柱速度为1.5mL/min,上样浓度为选择4 mg/mL,柱容量为2BV的条件下,多糖的脱色率为82.37%,多糖保留率为79.12%,蛋白去除率为88.39%。

By studying the impact of six macroporous resins and two ion exchange resins on decolorization of ginkgo polysaccharide pigment,three resins were selected: Macroporous anion exchange resin decoloration No.1,D900 and the non-polar macroporous adsorption resin DA-201C.Optimization of decoloration experiments were carried out by orthogonal experiment.Based on the static adsorption experiment,better resins were then chosen for dynamic experiment.The results showed that ginkgo polysaccharide pigment was mainly non-polar small molecules with negative charged.Using Decoloration No.1 resin,under the conditions of pH value 4.5,25 ℃,the columns speed 1.5mL/min,the concentration of the sample 4mg/ml and the column capacity of 2BV,the decolorization rate of the polysaccharide was 82.37%,the retention rate of the polysaccharide was 79.12% and the protein removal rate was 88.39%.