通过比较5种大孔吸附树脂对核桃壳总黄酮的吸附解吸性能,筛选出NKA-9树脂较适合纯化核桃壳总黄酮,并对其进行动态吸附特性研究。所确定优化工艺参数为:上样浓度1.0 mg/mL,pH值5.0,上样流速1.0mL/min,5BV体积分数95%乙醇洗脱效果最佳。核桃壳粗提物中总黄酮纯度为3.58%,经NKA-9树脂纯化后总黄酮纯度为62.3%,回收率达88.9%。

In this paper,five macroporous adsorption resins were chosen to compare their adsorption and desorption performances for flavonoids from Walnut Shell by using static adsorption experiment.The best performance resin was NKA-9.Its dynamic adsorption performance was further studied.The excellent technological parameters were: sample pH value 5.0,velocity 1.0 mL/min,concentration 1.0 mg/mL and 5BV 95% ethanol.The purity of flavonids was 3.58% in the crude extracts from walnut shell,and increased to 62.3% after purification with NKA-9 resin.The coefficient of recovery was 88.9%.