为了研究生姜中有效成分姜辣素、姜黄素以及生姜蛋白酶同步提取的方法,文中使用乙醇溶剂法结合旋蒸确定了生姜中3种有效成分同步提取的工艺条件,即生姜与无水乙醇料液比1∶3(g∶mL),生姜蛋白酶提取中缓冲液pH值为6.5,冰浴时间7 h;姜辣素、姜黄素旋转蒸发最佳温度为60℃,转速为16 r/min。在此优化条件下,生姜中姜辣素、姜黄素的提取率分别为0.3143%、0.3889%;生姜蛋白酶的得率为1.376%,比活力达到4 465 U/mg。此工艺能够同步提取姜辣素、姜黄素以及生姜蛋白酶3种有效成分,减少了原材料的消耗,降低生产成本,更适合产业化生产。

In order to study the effective extraction of ginger oleoresin,ginger protease and curcumin simultaneous extraction,ethanol solvent combined with rotary evaporation was studied.The ratio of ginger and ethanol liquid ratio was 1∶ 3 g∶ mL),extraction buffer pH 6.5,ice bath time 7h;for the ginger oleoresin and curcumin rotary evaporation,the optimum temperature was 60℃,the rotating speed was 16 r/min.Under the above optimized conditions,gingerol in ginger,curcumin extraction rate was 0.314 3% and 0.388 9%;ginger protease ratio was 1.376%,specific enzymatic activity reached to 4 465 U/mg.This process can simultaneously extract gingerol,curcumin and ginger protease three effective components,reduce the consumption of raw materials,the production cost,and therefore more suitable for industrial production.