从藏灵菇中分离纯化5株乳酸菌,初步鉴定2株为嗜酸乳杆菌,3株为乳酸乳球菌。选取其中2株菌研究其益生特性。结果表明,从藏灵菇中分离出的乳酸菌具有良好的益生特性,2株乳酸菌在pH值为4～6可生长良好;耐热范围为30～60℃;胆盐耐受性为0.1%～0.5%;发酵液对金黄色葡萄球菌、沙门氏菌、大肠杆菌等肠道病原菌有抑制作用;对抗生素有不同程度的耐药性。

Five strains of lactic acid bacteria were isolated from tibet kefir.Two strains were identified preliminarily as Lactcbacillus acidophilus and three strains were identified preliminarily as Lactococcus lactis.Two strains of lactic acid bacteria were selected to study their probiotic properties.The results showed that two strains of lactic acid bacteria had good potential probiotics.Two strains of lactic acid bacteria could grow well at pH 4~6.Their high temperature resistance range was from 30 to 60℃.Bile tolerance was 0.1%~0.5%.Fermentation broth could inhibit intestinal pathogens and had different resistance against antibiotics.