Flavonoids in Sweet-scented osmanthus are abundant,and have diversified biological activities.The extraction rate of total flavonoids was determined by orthogonal design and the patterns of dynamic accumulation of total flavonoids in Sweet-scented osmanthus collected from different harvest times were investigated.The results showed the optimum extractions were: A3B3C3D2.There is a fluctuation between the total contents of flavonoids in Sweet-scented osmanthus collected on September 13th and September 22th.The highest yield of total flavonoids was obtained on September 19th.It will provide a reference to the development of Sweet-scented osmanthus.