采用响应面法优化乙醇注入-动态高压微射流法制备鱼油纳米脂质体的工艺,并对其理化性质进行了初步测定。结果表明:制备鱼油纳米脂质体的最佳工艺为:磷脂浓度29 mg/mL,m(磷脂)∶m(鱼油)∶m(胆固醇)∶m(吐温-80)=10∶2∶2.5∶1,微射流压力150 MPa,微射流处理次数2次。在此条件下脂质体的包封率为76.9%,平均粒径128.1 nm,Zeta电位-20.11 mV。乙醇注入-动态高压微射流法制备的鱼油纳米脂质体粒径小且分布均匀(多分散指数0.258),具有较高的包封率和稳定性。

Response surface methodology(RSM) was selected to optimize the fish oil nanoliposomes prepare by ethanol injection-dynamic high pressure microfluidizer(DHPM) method,its physical and chemical properties were evaluated at last.The results indicated that the optimum prepation technique parameters were as follows: phosphatidylcholine content was 29 mg/mL,the mass ratio of phosphatidylcholine,fish oil,cholesterol and Tween-80 was 10∶ 2∶ 2.5∶ 1,DHPM treatment pressure was 150 MPa and treated twice.Under these conditions,encapsulation efficiency(EE) of fish oil nanoliposomes was reached to 76.9%,average diameter was 128.1 nm,zeta potential was-20.11 mV.Fish oil nanoliposomes made by ethanol injection-DHPM exhibited great advantages in their characteristics,including small average diameter and narrow size distribution(polydispersity index 0.258) as well as great encapsulation efficiency and good stability.