食品与发酵工业 >

2013 , Vol. 39 >Issue 02: 92 - 96

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2013.02.019

食品与发酵工业

海洋低温β-半乳糖苷酶菌株筛选、鉴定及酶的分离纯化

  • 崔爱萍 ,
  • 迟乃玉 ,
  • 张庆芳
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网络出版日期: 2013-02-25

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Isolation,identification and enzyme purification of a strain producing cold-activity β-galactosidase from marine

  • Cui Ai-ping ,
  • Chi Nai-yu ,
  • Zhang Qing-fang
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Online published: 2013-02-25

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摘要

筛选自黄海海泥产β-半乳糖苷酶的菌株CD6,依据形态学特征、生理生化特征及分子生物学特征鉴定为土生拉乌尔菌(Raoultella terrigena)。通过透析、超滤和柱层析方法分离纯化菌株β-半乳糖苷酶,测定该酶的最适反应温度为20℃,30℃剩余相对酶活85%;超过35℃酶活力即迅速下降,确定其为低温酶。

关键词： β-半乳糖苷酶; 鉴定; 分离纯化; 低温酶

本文引用格式

崔爱萍 , 迟乃玉 , 张庆芳 . 海洋低温β-半乳糖苷酶菌株筛选、鉴定及酶的分离纯化[J]. 食品与发酵工业, 2013 , 39(02) : 92 -96 . DOI: 10.13995/j.cnki.11-1802/ts.2013.02.019

Abstract

A strain CD6 from Huanghai sea mud was identified as Raoultella terrigena according to its morphology,taxonomical properties and molecular characterization.The purified β-galactosidase was obtained through dialysis,ultrafiltration,and column chromatography.Researches on the β-galactosidase showed that its optimal temperature was 25℃ and it was defined as cold-activity enzyme.

Key words： β-galactosidase; identification; purificayion; cold-activity enzyme

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技术支持：北京玛格泰克科技发展有限公司