Milk is nutritious and suitable for the growth of a variety of microorganism.This review introduces the types of microorganisms in raw milk.It focuses on the research progression of microbial diversity in raw milk in recent years.The application of Culture-independent microbiology on the study of microbial diversity in raw milk was summarized.Here,we will highlight the benefits associated with culture-independent methods which include enhanced sensitivity,rapidity and the detection of microorganisms not previously associated with such milk.