对体外模拟胃肠液中燕麦β-葡聚糖吸附表没食子儿茶素没食子酸酯(EGCG)的影响因素进行研究。结果显示:燕麦β-葡聚糖吸附EGCG受胃蛋白酶、胰蛋白酶、pH、EGCG浓度影响较大。加入胃蛋白酶和胰蛋白酶,燕麦β-葡聚糖对EGCG吸附量增大。模拟胃液中随pH值升高,β-葡聚糖吸附能力逐渐增强;肠液中随pH值升高,吸附能力首先升高然后下降,pH 6.5时,其吸附能力最大。随EGCG浓度升高,响应因子增多,也使β-葡聚糖对EGCG的吸附能力增强。

The influence factors of oat β-glucan on the adsorption of epigallocatechin gallate(EGCG) in vitro simulated gastrointestinal fluid were studied.The result showed that the oat β-glucan adsorption EGCG was more likely influenced by pepsin,trypsin,pH and EGCG concentration.The amount of EGCG adsorption increased if pepsin or trypsin was added to vitro simulated gastrointestinal fluid.The adsorption capacity of the β-glucan gradually increased with pH increasing in the simulated gastric fluid.In intestinal fluid,the capacity increased first and then declined with the pH increasing.The biggest adsorption capacity was at pH 6.5.With EGCG concentration increased,the response factors increased,so did the adsorption capacity of the β-glucan.