采用超临界CO2萃取技术提取柚子叶、花中的精油,运用气相色谱-质谱(GC-MS)联用技术对其化学成分进行分离和鉴定,并用峰面积归一化法确定各组分的相对含量。结果表明:从柚子叶精油中分离出61个色谱峰,鉴定出39个化合物,占总离子峰相对含量的90.05%。其主要化学成分有匙叶桉油烯醇(21.36%),石竹烯氧化物(17.16%),β-石竹烯(8.40%),α-石竹烯(2.07%),异芳萜烯氧化物(2.01%)等。从柚子花精油中分离出42个色谱峰,鉴定出27个化合物,占总离子峰相对含量的89.31%。其主要化学成分有金合欢醇(19.02%)、橙花叔醇(18.24%),龙脑(6.60%),芳樟醇(4.03%),橙花醇(2.57%)等。柚子叶与柚子花精油共同鉴定出的化合物有橙花醇、香叶醇、香叶酸、β-石竹烯等12种,其相对含量存在不同程度差异。

Essential oil of pomelo leaf and flower was extracted by supercritical CO2 fluid extraction technology.Their compositions and relative contents were quantitatively determined by GC-MS with peak area normalization method.Results showed that sixty-one chromatogram peaks from essential oil of pomelo leaf were isolated,thirty-nine volatile compounds had been identified,which was accounted for 90.05% of total volatile compounds.The predominant volatile components were isospathulenol(21.36%),caryophyllene oxide(17.16%),β-caryophyllene(8.40%),α-copaene(2.07%),isoaromadendrene epoxide(2.01%).Forty-two chromatogram peaks from essential oil of pomelo flower were isolated,twenty-eight volatile compounds had been identified,which was accounted for 89.31% of total volatile compounds.The predominant volatile components were farnesol(19.02%),nerolidol(18.24%),borneol(6.60%),linalool(4.03%),nerol(2.57%).Twelve chemical components such as nerol,geraniol,geraniol acid,β-aryophyllene were both presented in pomelo leaf and flower,but with different contents.