利用乙醇回流提取杨梅树叶中杨梅素。为优化提取工艺条件,在单因素实验基础上,采用三因素三水平的响应面分析法对各个因素的显著性和交互作用进行分析。结果表明:液料比对提取得率有极显著影响,乙醇体积分数对提取得率有显著影响,而提取温度、提取时间对提取得率的影响不显著;最佳提取工艺为提取温度84.86℃、乙醇体积分数82.09%、液料比为12.82∶1、提取时间1.5 h,在此条件下,杨梅素的提取得率可达1.355%。

The study was to optimize the alcohol reflux extraction of myricetin from myrica rubra leaves.The selection of extraction factors was investigated on the basis of one-factor-at-a-time(OFAT) experiments.Then,the significance of each factor and interaction analysis of dependent variables and independent variables were studied according to a three-variable,three-level central composite design.The results showed that material/liquid ratio had an extremely significant effect on myricetin yield,volume fraction of alcohol significantly affect myricetin yield,whereas extraction temperature and extraction time had no significant effect on myricetin yield.Under the conditions of : extraction 1.5h at 84.86℃,with 82.09% alcohol assistance and liquid/material ratio of 12.82,the maximum myricetin yield was reached to 1.355%.