以黑羽乌骨鸡毛为原料,采用盐酸水解法从乌骨鸡毛中提取黑色素并对黑色素的含量进行测定。通过单因素实验考察了提取时间、盐酸浓度、料液比对乌骨鸡毛中黑色素得率的影响,在单因素实验基础上通过响应面实验分析法得到二次多项式回归方程的预测模型。结果表明:乌骨鸡毛中黑色素提取的最佳工艺条件为,HCl浓度27%,提取时间6h,料液比(g∶mL)1∶2,在此条件下黑色素得率达4.72%。黑色素样品经过氧化氢法氧化处理,采用高效液相色谱法测出黑色素含量为76.20%。

In order to optimize acid extraction of black feather silky fowl.Method: Black feather silky fowl was used as raw material,hydrochloric acid hot reflux was applied in extracting silky fowl melanin.The melanin content was also determined.Three independent variables affected yield of melanin: extraction time,hydrochloric acid concentration and solvent/material ratio.On the basis of single factor experiment and the response surface methodology,the best conditions of extraction were determined.Then the mathematical regression model was used to predict the extraction yield of melanin.Result: The optical protocol was: 27% hydrochloric acid,extraction 6h,material to solvent ratio of 1∶ 2(g·mL-1).Under the above conditions,the extraction yield was up to 4.72%.The sample of melanin was processed by hydrogen peroxide oxidation treatment and tested by high performance liquid,the content was 76.20%.Conclusion: The hydrochloric acid hydrolysis method can be used in extracting the melanin of black feather silky fowl.