为优化南极磷虾粉中虾青素的提取方法,在单因素实验基础上,选取提取温度、料液比、提取时间为自变量,虾青素含量为响应值,采用中心组合(Box-Behnken)实验设计方法,研究各自变量及其交互作用对虾青素含量的影响。利用Design-Expert软件,建立了虾青素含量与提取过程中各因素的二次多项式模型,并通过响应面优化法确定虾青素提取方法的最佳条件:提取温度51.41℃、料液比63.13∶1(mL∶g)、提取时间61.15 min,此条件下虾青素含量达到最大为179.00 mg/kg。经过实验验证,南极磷虾粉中虾青素含量可达到179.21 mg/kg。

To optimize extraction method of astaxanthin from Antarctic krill powder.Temperature,liquid/soild ratio,extraction time were selected as the independent variables.The yield of astaxanthin content was used as response value to study the effects of each factor as well as their interactions on the yield of the astaxanthin by central composite design.The predictive polynomial equations model was developed by design expert software.Response surface methodology was used to optimize the experimental conditions.The results showed that the optimum conditions of astaxanthin extraction were as follows:extraction temperature 51.41℃,liquid/soild ratio 63.13 mL/g,extration time 61.15 min,and the optimum astaxanthin content was 179.00 mg/kg.After experimental verification,astaxanthin content could reach 179.21 mg/kg.By studying the extraction and determination of astaxanthin in Antarctic krill powder,we selected an efficient and practical extraction and separation process.A simple,accurate determination of astaxanthin method was also established.