制成了固定化酶化学发光型葡萄糖传感器,此传感器可用于对葡萄糖的在线快速检测。用MCM-41介孔分子筛固定葡萄糖氧化酶(GOD),制成GOD酶柱,并用于流动注射化学发光分析。酶柱的使用条件为:柱温40℃,葡萄糖溶液pH=6.0,流速3 r/min。测定的线性范围为1～200 mg/L,检出限为0.2 mg/L,10次测定的RSD为1.8%,回收率在97.81%～102.0%。测定结果与国标方法的测定结果无显著性差异。

In this paper,immobilized enzyme chemiluminescence glucose sensor was made,the sensor can be used for rapid detection of glucose.GOD enzyme column was prepared through the immobilization of GOD with MCM-41 mesoporous molecular sieves and then used in flow injection chemiluminescence analysis system.The optimum condition for GOD column was reaction temperature of 40℃,glucose solution of pH=6.0,velocity of 3 r/min.The liner range of glucose concentration was from 1 to 200 mg/L.The detection limit was 0.2 mg/L.The relative standard deviation(RSD) was 1.8%(n=10),and the recoveries were 97.81%-102.0%.The results showed that the method possessed many advantages such as saving enzyme reagent,simply handling,easy operation,high specificity and rapid determination.There was no significant difference between the determination results obtained through this method and through the standard method.