以珠蚌多肽为原料,研究超声波改性珠蚌多肽与钙的螯合能力,并对改性多肽与肽钙螯合物的结构进行初探。研究表明:超声波改性珠蚌多肽的最佳工艺为,超声功率160 W、超声时间10 min、超声温度50℃、液固比40∶1(mL∶g),此时钙质量分数达到6.037%。通过FTIR、UV、SEM等技术对改性多肽及其肽钙进行分析,FT-IR分析得出羧基参与肽钙螯合反应生成羧酸盐;UV分析得出改性多肽与钙离子通过配位键结合形成肽钙螯合物;SEM分析表明改性肽钙的结构类似于盐类。

The influence of ultrasonic pretreatment on modified oyster peptide binding calcium capacity during oyster peptide process was studied.The structural characterization of the modified peptide chelating calcium was also studied using the spectra technologies.The results showed that the optimum processing of modified peptide were as follows: 80% of ultrasonic power,ultrasonic time of 10min,ultrasonic temperature 50 ℃,liquid-solid ratio 40:1.Under the above conditions,the quality score of calcium is 6.037%.FT-IR,UV and environmental scanning electron microscope(SEM) were used to study the characterization of the modified peptide and modified peptide calcium.FI-IR spectrum indicated that the carboxyl group can participate in the reaction to form carboxylate.UV spectrum indicated that the peptide and calcium chelate was formed by modified peptide and calcium through coordination bonds.SEM showed that microstructure of modified peptide and calcium chelate was similar to that of salt.