建立了基于T/C比值的胶体金免疫层析法快速定量检测猪肉中克伦特罗残留的新方法。胶体金免疫层析试纸条的定量检测线性范围为0.1～1.5 ng/g,检测猪肉中克伦特罗残留的最低检测灵敏度为0.19 ng/g。比较了5种猪肉组织样本中克伦特罗的简便提取方法,其中采用0.02 mol/L,含2.8%NaCl的HCl溶液抽提猪肉样品2次的提取方案,克伦特罗的平均回收率达到76.7%,变异系数为7.4%。实际样本加标回收实验显示,加标量为0.5、1.0、2.0及3.0 ng/g时,胶体金试纸条检测回收率分别为(60.4±12.8)%,(70.24±4.2)%,(75.9±4.9)%,(71.1±5.0)%。与传统ELISA方法比对,结果显示2种方法具有较好的相关性(R2=0.9136)。以上实验结果证实,基于T/C比值法的胶体金免疫层析试纸条可用于猪肉组织样品中克伦特罗的快速及定量检测。

Based on T/C ratio,a colloid gold immunochromatographic assay was developed for rapid and quantitative detection of clenbuterol residues in pork.The linear range of the new quantitative method was from 0.1 to 1.5 ng/g with a detection limit at 0.19 ng/g.Five pretreatment methods were compared.The result showed the best condition was 0.02 mol/L HCl containing 2.8% NaCl as the extracting solvent,extracting twice.The average recovery was 76.7% with a coefficient of variation 7.4%.For real sample spiked experiments,the results showed that the average recoveries were 60.4±12.8%,70.24±4.2%,75.9±4.9% and 71.1±5.0% with CLE concentrations of 0.5,1,2 and 3 ng/g,respectively.The new quantitative system had a high coefficient of correlation with traditional ELISA(R2=0.9136).These results demonstrated that T/C ratio based colloidal gold test strip is a sensitive,reproducible method for on-site detection of CLE in pork samples.