建立快速检测蜂蜜中有毒物质5-羟甲基糠醛(HMF)的胶体金免疫层析试纸条。文中采用免疫竞争法,将制备的胶体金颗粒与抗HMF单克隆抗体结合成抗HMF-金标复合物标记在胶体金结合垫上,并将HMF-载体蛋白包被在硝酸纤维素膜上作为检验线(T线),其与待测蜂蜜中HMF竞争结合胶体金标记的抗HMF单克隆抗体,并能以颜色直观显示检测的定性结果。结果显示:若蜂蜜液中HMF的含量<40 mg/kg,则过量的胶体金标记物与T线上HMF-载体蛋白特异性结合形成紫红色条带,即阴性;若蜂蜜液HMF含量≥40 mg/kg,不能产生紫红色条带,即阳性。该方法是对分光光度法及HPLC法测定蜂蜜中HMF含量的补充,并且该法灵敏度高,操作简便快速等优点。

To develop a rapid,simple detection of colloidal gold immunochromatographic assay for detecting the toxic substance 5-Hydroxymethy furfural(HMF) in honey.Methods: competing antibody binding reaction was applied.Nanocolloidal gold particles were prepared and labeled to anti-HMF monoclonal antibody.Anti-HMF was conjugated to bovine serum albumin(BAS) and then dispersed on a nitrocellulose membrane as the test line(T).The presence or absence of a colored bang on the test line indicated a negative or positive result.Result: If the content of HMF in honey extract is less than 40mg / kg,then the excess of colloidal gold-labeled complex with T line HMF-specific binding of carrier protein to form purple bands to form a negative band.Otherwise,it means positive.Conclusion: This method is an alternative method for the test of HMF content in honey extract using spectrophotometry and HPLC.The method is also sensitive,simple and rapid.