建立了一种用高效液相色谱(HPLC)检测苹果树腐烂病菌发酵液中有机酸和葡萄糖的方法。采用Atlantis C18色谱柱(150 mm×4.6 mm,3.5μm),以5g/L NH4H2PO4(用磷酸调pH=2.5)溶液为流动相,流速为0.6mL/min,柱温30℃,应用紫外检测器检测有机酸,示差检测器来分析葡萄糖。在此色谱条件下,葡萄糖和有机酸能很好地被分离检出,平均回收率为96.2%~102.2%,精密度RSD(n=5)为0.26%~0.75%,每一样品的分析时间不超过15 min。该方法能够简便、快速测定苹果树腐烂病菌发酵体系中有机酸及葡萄糖的含量。

A method for determination of organic acids and glucose in fermentation broth of Valsa mali var.mali by HPLC was established.The Atlantis C18 column(150 mm × 4.6 mm,3.5 μm) was used.Mobile phase was 5 g / L NH4H2PO4(pH2.5).The flow rate was 0.6 mL/min,and the temperature of column was 30℃.Organic acid and glucose in samples were determined by ultraviolet visible detector(UV) and refractive index detector(RID),respectively.They were separated effectively at the chromatographic condition.The average recovery was in the range from 96.2% to 102.2%,and the relative standard deviation(RSD,n = 5) was in the range from 0.26% to 0.75%.By this method,organic acids and glucose can be easily,rapidly and effectively determined within 15 minutes.