采用正交试验设计对野鸢尾植物中总黄酮的提取工艺进行了优化。结果表明,影响野鸢尾总黄酮的优化提取工艺因素为提取温度>乙醇浓度>料液比>提取时间;优化提取工艺条件为A2B2C3D1,即乙醇体积分数为80%,料液比1∶20(g∶mL),提取时间为5 min,提取温度为90℃,此条件下总黄酮为4.007 mg/g。

For the first time,the extraction optimization of total flavonoids in iris dichotoma optimized by orthogonal experiments is reported.The results showed that the order of the factors influencing the optimum extraction technology was: ethanol concentration > extraction temperature > ratio of solid to liquid > extraction time.The best extraction technology conditions was A 2 B 2 C 3 D 1,80% ethanol concentration,ratio of solid to liquid 1∶ 20,extraction time 5 min,extraction temperature 90℃,the total flavonoids was 4.007 mg / g.The experiment provided scientific basis of the development and utilization of iris dichotoma flavonoids.