优化双花千里光总黄酮的提取工艺,并对其总黄酮粗提物进行抗氧化活性研究。在单因素实验的基础上,利用正交实验优化总黄酮最佳提取工艺,考察提取温度、提取时间、液固比、乙醇浓度对总黄酮含量的影响。利用Fenton反应和Smirnoff的方法分别测定总黄酮还原能力和清除羟基自由基能力。结果显示:总黄酮的优化提取工艺是,提取温度90℃、提取时间2 h、液固比30∶1(mL∶g)、乙醇体积分数70%。总黄酮粗提物在一定浓度内具有较强的清除羟基自由基能力。

To optimize the extraction of total flavonoids from Senecio dianthus Franch.and determine their antioxidant activity.Using an orthogonal design with single-factor experiments,the effects of refluxing temperature,extraction time,liquid-solid ratio and ethanol concentration were investigated to optimize the extraction.The reducing effect of total flavonoids and ability to eliminate ·OH were also investigated by the method of Fenton and Smirnoff.The optimum extraction conditions were as follows: reflux temperature 90 ℃,extraction time 2 h,liquid-solid ratio 30 ∶ 1,and ethanol concentration 70%.The flavonoids extracts in the scavenging ·OH was highly effective in the certain concentration range.The extracting method was optimized,and the total flavonoids had strong antioxidant activity in the certain concentration range.