以玫瑰果实为原料,采用乙醇浸提法提取玫瑰果多酚类物质。通过单因素及正交试验研究了不同的乙醇体积分数、固液比、提取温度、提取时间和提取次数对多酚得率的影响。结果表明:各因素对玫瑰果多酚得率影响的主次顺序为乙醇体积分数>提取温度>料液比>提取时间;优化提取工艺条件为乙醇体积分数75%,固液比1∶20(g∶mL),浸提温度70℃,提取时间40 min,提取2次。在该优化条件下提取,玫瑰果多酚得率达到9.18%。
关键词:
玫瑰果; 多酚; 提取; 测定; 单因素
Alcohol steeping-extraction technology was used to extract polyphenols from roses.The single factor experiments and orthogonal test were adopted to study the impacts of different alcohol concentration,material / liquid ratio,extraction temperature,extraction duration and extract times.Experimental results showed that the order of different effect factors on yield was : alcohol concentration > extraction temperature > materiel / liquid ratio > extraction duration;the optimal extraction process is: 75 % alcohol,1: 20material / liquid ratio,70 ℃ extraction temperature,extraction for 40 min,and extract twice.The pure yield of polyphenols under the above extraction condition was 9.18 %.The method is easy to operate,low-cost and highly efficient.It is suitable for the massive industrial production.