为建立不同温度下鲜切结球莴苣中单增李斯特菌生长模型,将单增李斯特菌接种到鲜切结球莴苣表面,并于不同温度下贮藏,获得其在4、8、16、24和32℃下的生长数据,选用Gompertz模型进行拟合,建立初级生长模型。在此基础上建立二级模型研究温度对初级模型中单增李斯特菌生长动力学参数的影响,并进行数学检验。结果表明,对最大比生长速率和延滞时间建立平方根模型,结果呈良好的线性关系,相关系数R2分别为0.977 2和0.984 7,所建立的预测模型能很好地描述不同温度下单增李斯特菌的生长动态。

This paper was designed to establish the growth model of Listeria monocytogenes in fresh-cut iceberg lettuce in different temperatures.The L.Monocytogenes was inoculated to the surface of fresh-cut iceberg lettuce and then preserved in temperature of 4 ℃,8 ℃,16 ℃,24℃,32 ℃.Gompertz model was selected as the primary model.A secondary model was created to study the effects of temperature on growth kinetics parameters of Listeria monocytogenes in the primary model.Mathematical evaluation was also conducted.The results showed that the regression of Belehradek model well described the effect of temperatures on the maximumgrowth rate and the lag phase,and correlation coefficients of determination were 0.9772,0.9847,respectively.Therefore,the model can predict the growth of L.monocytogenes in fresh-cut iceberg lettuce perfectly.