以小麦胚芽蛋白为原料,经蛋白酶酶解后与Ca2+键合形成肽钙络合物,可作为第4代钙补充剂。碱性蛋白酶具有更高的麦胚蛋白水解活性,是制备高键合钙能力水解液的最适合酶类,且水解度在21.5%时钙的结合量达最大值18 mg/g蛋白;SDS-PAGE表明碱性蛋白酶迅速降解麦胚蛋白中高分子量亚基,且50 kDa和32~34 kDa亚基的水解与钙离子键合能力密切相关;键合钙能力强的水解产物中主要含Glu、Arg、Asp、Gly,且含44%疏水性氨基酸;分子量分布显示键合钙的小肽分子量在2 000 Da以下;全波长扫描和红外图谱表明肽段上的氨基和羧基均参与了键合反应,并生成了新的物质——麦胚肽钙络合物。

In this paper,wheat germ protein(WGP) as raw material was hydrolyzed by protease and bond to Ca2+.The formation of wheat germ peptide-calcium complex can be the fourth-generation calcium supplements.Alcalase could hydrolyze wheat germ protein more easily.The combined amount of calcium reached a highest value of 18mg / g protein at the degree of hydrolysis of 21.5%.Result of SDS-PAGE showed the quick degradation of the high molecular weight subunit in WGP,and showed that the hydrolysis of 50kDa and 32 ~ 34 kDa subunit was intimately related to calcium binding capacity.The hydrolysates with high calcium binding ability mainly contained Glu,Arg,Asp,Gly and 44% of hydrophobic amino acids,in which the molecular weight distribution was lower than 2 000Da.Results of full wavelength scanning and infared spectra revealed that the amino and carboxyl groups were involved in the binding reaction and generated new material-wheat germ peptide-calcium complex.