对蝇蛆壳聚糖的柱层析分级纯化和一级结构进行了研究。采用SephacrylTMS-300凝胶层析分离纯化碱液法提取的蝇蛆壳聚糖(脱乙酰度为79.45%),分离得到分子质量分别为230和620 kDa的2个组分,经纯度的测定分析及高效液相色谱分析,所得组分纯度较高,分别为98.5%和98.2%。对分级后2个组分进行了红外光谱分析和晶型结构分析,结果表明:在红外光谱图中两组分均出现了壳聚糖官能团的特征吸收峰;组分1结晶度不高,组分2结晶度较高。

ephacryl S-300 gel filtration chromatography was used to purify Chitosan from Maggots shell.The Chitosan was identified as two fractions by HPLC analysis,the molecular weight was 230 and 620 kDa,respectively.The results indicated that Sephacryl S-300 chromatography was useful for purification of Chitosan.The two fractions was analyzed by infrared spectrum and X-ray,the results showed that both fractions had characteristic absorption peak of Chitosan functional group in the IR spectrum.Crystallinity degree of fraction one was low and the fraction two was high.