以缺刻缘绿藻(Parietochloris incisa)为试验材料,采用差量法、超声波萃取法、气相质谱仪分析法和分光光度法等方法,研究了氮源和光照强度对缺刻源绿藻生长、总脂、花生四烯酸和色素含量变化的影响。结果表明:无氮源,35μmol photons/(m2·s)低光强下可降低缺刻源绿藻的生长速度和生物量。而在有氮源、280μmol photons/(m2·s)高光强下,缺刻源绿藻的生物量达到最大值,为7.23 g/L。无氮源、光强度均能不同程度的提高藻细胞总脂肪及花生四烯酸的含量,且在无氮源、280μmol photons/(m2·s)高光强条件下油脂量最高,约为38.02%,在缺氮源、135μmol photons/(m2·s)中光强条件下花生四烯酸含量最高,约为16.10%。色素含量在缺氮条件下随着光强的上升而下降,在有氮源、135μmol photons/(m2·s)中光强条件下达到最大值,约为150.00 mg/L。类胡萝卜素含量无论是在有氮源还是无氮源培养基中,135μmol photons/(m2·s)中光强条件均较高。

The effects of nitrogen and different light intensity(35 μmol photons /(m2·s),135μmol photons/(m2·s),280μmol photons/(m2·s)) on the growth,total fatty acid(TFA),arachidonic acid(AA) and pigment composition of Parietochloris incisa was investigated.The results suggested that the cultures showed relatively slow increase in biomass under nitrogen-sufficient and low(135μmol photons /(m2·s)) light intensity.The nitrogen-sufficient and high light intensity [280 μmol photons /(m2·s) ]culture reached the highest biomass at the average rate of 7.23 g / L.An increase in TFA was in company with the accumulation of AA in nitrogen-sufficient medium and certain light intensity.The highest percentage of TFA and AA was 38.02% and 16.10% in the light intensity of 280 μmol photons /(m2·s) or 135μmol photons/(m2·s),respectively.Under nitrogen-sufficient culture,carotenoids content was considerably lower relative to that observed during cultivation on complete medium and did not exceed 9.60 mg / L in all these cases.The highest chlorophyll content reached 150.00 mg / L in the nitrogen-sufficient medium and moderate irradiance cultures.