为了掌握江苏啤酒大麦制麦过程中细菌的群落结构,利用传统培养结合PCR-DGGE的方法对江苏单二大麦制麦过程中的细菌群落进行了分析。结果表明,细菌数量在浸麦、发芽阶段呈不断上升趋势,发芽结束时达到峰值,随着排潮、焙焦的进行,细菌数量有所下降,但与原始大麦相比仍有增加。制麦过程中检测到11个属的细菌,主要有Acinetobacter,Enterobacter,Erwinia,Flavobacterium,Kluyvera,Lactobacillus,Lactococcus,Leuconostoc,Pantoea,Streptococcus,以及Wautersiella。

The objective of this study was to investigate the bacteria communities during the malting of Jiangsu malting barley by the methods of traditional culture planting combined with PCR-DGGE.The results showed that the number of bacteria increased during steeping and germination period,which reached the peak in the end of germination.Furthermore,bacteria growth was decelerated in the kilning period,but the viable counts of bacteria were generally higher in the malt than in native barley.Results showed that total of eleven bacteria species were detected,including Acinetobacter,Enterobacter,Erwinia,Flavobacterium,Kluyvera,Lactobacillus,Lactococcus,Leuconostoc,Pantoea,Streptococcus,and Wautersiella.