采用种子表面灭菌、菌种分离与纯化、ITS rDNA和26S rDNA D1/D2区序列测定和系统发育分析方法,对西沙野生诺尼种子内生真菌群落多样性进行了初步研究。从诺尼种子中共分离到内生真菌48株,其中酵母菌39株,霉菌9株;39株酵母菌分属于4个属内的5个种,其中榛针孢酵母(Eremothecium coryli)、Pseudozyma aphidis和浅黄隐球酵母(Cryptococcus flavescens)为优势种,分别占总菌株数的31.25%、27.08%和16.67%;9株霉菌分属于4个属内的4个种,其中Phaeoacremonium sp.和赤霉属(Gibberella sp.)是霉菌中相对优势的种,分别占总菌株数的8.33%和6.25%。研究结果表明,西沙野生诺尼种子中存在大量内生真菌,且种类丰富,其中酵母菌占绝对优势,部分优势种具有潜在的药用价值。

Through leaf surface disinfection,strain isolation and purification,ITS rDNA and 26S rDNA D1 /D2region sequence determination and phylogenetic analysis,a priliminary study on the diversity of culturable entophytic fungus community in seeds of Xisha wild Noni was conducted. 48 entophytic fungus strains were isolated,among which39 strains were yeasts,9 strains were molds. 39 yeast strains were belong to 5 species in 4 genera. The dominant yeasts( abundance) were Eremothecium coryli( 31. 25%),Pseudozyma aphidis( 27. 08%) and Cryptococcus flavescens( 16. 67%). 9 mold strains belonged to 4 species in 4 genera. The dominant molds( abundance) were Phaeoacremonium sp.( 8. 33%) and Gibberella sp.( 6. 25%). The results indicated that there was a significant diversity in culturable entophytic fungus community in seeds of Xisha wild Noni and the dominant entophytic fungus were yeasts.Parts of those strains had potential pharmaceutical value.