以甜菊糖苷的吸附量、解吸率、回收率和纯度等为指标,进行了氧化铝及多种大孔吸附树脂分离纯化甜菊糖苷的试验。静态筛选试验显示:AB-8与ADS-7二种大孔吸附树脂适用于甜菊糖苷的柱分离;动态试验显示,ADS-7比AB-8对甜菊糖苷的纯化效果更好,ADS-7的饱和吸附量为184.29 mg/g,解吸率为81.66%,回收率为74.12%,产物甜菊糖苷的纯度可达96.95%,是柱分离法制备高纯度甜菊糖甙的最佳分离树脂。

The separation and purification of steviosides by column chromatograph was studied. Adsorption capacity,desorption ratio,recovery ratio and purity were chosen as indexes,column separation method with alumina and many kinds of macroporous adsorption resin as adsorbent was performed. Static tests indicated that two kinds of macroporous adsorption resin,AB-8 and ADS-7,were suitable. However,dynamic testing showed the effect of ADS-7 was better than that of AB-8. The saturated adsorption capacity,desorption ratio and recovery ratio of ADS-7 were184. 29 mg / g,81. 66% and 74. 12%,respectively. ADS-7 was showed to be the best separation resin for preparing high purity stevioside as high as 96. 95% in the final product.