以酪丁酸梭菌为出发菌株,葡萄糖为底物进行批次和补料发酵生产丁酸。基于5 L通用发酵罐和纤维床生物反应器(FBB)发酵生产丁酸的研究基础,在5 000 L体系纤维床上进行丁酸发酵的中试规模放大。5L通用发酵罐发酵体系中,固定化批次发酵的平均糖酸转化率为0.47 g/g,丁酸生产强度为0.82 g/(L·h),分别比游离发酵提高了31%和134%。补料发酵中,丁酸浓度达52.8 g/L,比游离补料发酵提高了46%。在纤维床中试规模放大研究中,批次发酵的平均糖酸转化率为0.48 g/g,丁酸生产强度为0.63 g/(L·h),比游离发酵分别提高了37%和67%。补料发酵的丁酸浓度为51.63 g/L,与游离发酵相比提高了40%。结果表明,与实验室规模相比,纤维床反应器能够放大到1 000倍,能够保持较高的丁酸发酵水平和生产效率。

In this paper,efficient production of butyric acid from glucose by Clostridum tyrobutyricum was performed with batch and fed-batch modes. Based on the results of 5-L fermentor's experiments,the fermentation process was successfully scaled up in a 5000-L fermentor. In 5-L fermentor batch modes,butyrate yield and productivity was reached to 0. 47 g / g and 0. 82 g / L·h respectively,which was improved by 31% and 134% compared with fermentations of free cells. In the fed-batch fermentation,the final concentration of butyric acid was 52. 8 g / L,increased by 46% compared to free-cell fermentation. From up-scaled fed-batch production of butyrate in the pilot plant,butyrate yield,productivity and butyrate concentration with immobilized-cells reached 0. 47 g / g,0. 63 g /( L·h) and 51. 63 g / L,which were improved by 37%,67% and 40% respectively. Results showed that compared with the laboratory scale,the fibrous bed reactor could maintain a higher level of butyric acid fermentation and the production efficiency when zoom up to 1000 times. The present work provides a powerful alternative to produce butyric acid as a bulk chemical in pilot scale.