以茯砖茶为菌源,分离筛选出20株具有3-苯氧基苯甲酸(3-PBA)降解能力的"金花菌",其中分离的菌株ET1可在7d内完全降解PD培养基中100 mg/L的3-PBA,根据其形态特征和ITS序列分析(GenBank Accession No.KF317836),将其鉴定为冠突散囊菌(Eurotium cristatum)。动力学研究表明,菌株ET1降解3-PBA的过程符合一级动力学方程,在测试的3-PBA质量浓度、温度、pH值范围内,3-PBA半衰期为1.7~3.2 d,远远低于其自然降解半衰期(180 d),且该反应的速率受pH值影响较大,受3-PBA质量浓度和温度的影响相对较小。

Adopting fuzhuan tea as bacteria source,twenty strains of 3-phenoxybenzoate acid( 3-PBA) degrading"golden flower fungi"were screened. Strain ET1 isolated from the fuzhuan tea could completely degrade 100mg/L 3-PBA in the PD medium in 7d. Based on the analysis of morphological characteristics and its sequence( GenBank Accession No. KF317836),strain ET1 was identified as Eurotium cristatum. Kinetic research indicated that the degradation of 3-PBA by strain ET1 could be described by a first-order kinetics model. The degradation half-life of 3-PBA was 1. 7 to 3. 2d over a certain range of 3-PBA concentration,temperature and pH,which was much shorter than its natural half-life. The pH were found to influence the reaction rate obviously but the concentration of 3-PBA and temperature had little effect on reaction rate.