为研究蜂花粉中酚类物质的分布及其抗氧化活性,文中以槭树蜂花粉、蒲公英蜂花粉和山里红蜂花粉为研究对象,测定其游离态、酯键合态、糖苷键合态和甲醇不溶态酚类物质中总酚酸和总黄酮的含量,以及对Fe3+的还原能力和清除DPPH·的能力。结果表明:这3种蜂花粉中酚类物质的分布不同,以游离态和酯键合态形式存在的酚类物质多,抗氧化活性强,而以糖苷键合态和甲醇不溶态形式存在的酚类物质少,抗氧化活性弱。此外,3种蜂花粉中酚类物质含量不同,槭树蜂花粉最高,山里红蜂花粉次之,蒲公英蜂花粉最低。经比较分析3种蜂花粉抗氧化活性大小顺序为:槭树蜂花粉>山里红蜂花粉>蒲公英蜂花粉。

The aim of this study was to investigate the distribution of phenolic compounds in bee pollen and their antioxidant activity. Total phenolic content,total flavonoid content,reducing power and DPPH free radical scavenging activity of four states of the extracts( free,esterified,glycosided,and insoluble) of Aceraceae,Taeaxacum and Crataegus pinnatifida bee pollen were determined. The results showed that distribution of phenolic compounds in three kinds of bee pollen was different. Phenolic compounds free state and esterified sate were higher than in glycosided and insoluble state. Therefore,the antioxidant activity of the former was greater than the latter. In addition,the content of phenolic compounds in different bee pollen was different. Phenolic compounds in bee pollen of Aceraceae were higher than others. The order of antioxidant activities of three kinds of bee pollen was Aceraceae > Crataegus pinnatifida >Taeaxacum.