The ultrasound-assisted water extraction process of flavonoids from Eucommia ulmoides leaves was studied. Through single factor and orthogonal experiment,solid-liquid ratio,ultrasonic power,extraction time and temperature on the extraction rate were investigated. The results showed that: under the conditions of solid-liquid ratio1∶ 35,ultrasonic power 80W,extraction time 20min,extraction temperature 50 ℃,flavonoids extraction rate could reach 8. 45%. This process can shorten the extraction time and lower the energy cost. It was also 0. 96% and 0. 68%higher than the conventional water extraction and icrowave-assisted water extraction respectively.